Analysis Note

ControlMouse brain tissue, Astrocyte culture.

Application

Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 is an antibody against Glial Fibrillary Acidic Protein for use in IC, IH, IH(P) & WB with more than 55 product citations.

Research Sub CategoryNeuronal & Glial Markers

Research CategoryNeuroscience

Immunocytochemistry:
5 µg/mL of a previous lot was used.

Immunohistochemistry:
5 µg/mL

Immunoblotting:
Recognizes a 51kDa protein in reducing westerns of total brain lysates.

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.

Immunogen

Purified glial filament (Debus, E., 1983).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1031; 04-1062

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Purified mouse monoclonal IgG1 in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide, pH 7.6.

Format: Purified

Quality

Routinely evaluated by Western Blot on Mouse brain lysate.

Western Blot Analysis:
1:1000 dilution of this lot detected Glial Fibrillary Acidic Protein on 10 µg of Mouse Brain lysate.

Specificity

The antibody reacts with GFAP from human, pig, chicken and rat. In tissue sections this antibody stains astrocytes and Bergman glia cells (Debus, E., 1983).

Expected to cross-react with porcine, chicken, bovine, and rabbit.

Storage and Stability

Store the reconstituted antibody at 2-8°C for up to 6 months after date of receipt.
DO NOT FREEZE.

Target description

50 kDa